Mix & Go! E. coli Transformation Kit by Zymo Research

"The transformation process is very efficient and extremely time-saving. It barely takes 5 minutes for the transformation process. Insanely amazing. Traditional methods are time-consuming and require more equipment and materials. The efficiencies are the same for both, and I would say better than the traditional transformations protocol. Our students save time and use this to learn more about science and other related things. Also, when we explain to students to compare these methods, they say why has it taken so long for this to become so efficient and time-saving. We said that is to the credit of the Zymo research scientist and their research and development teams. All other products from Zymo are efficient and easy to use. We at Yard science and the students benefit a lot from their products. Thank you Zymo research for the wonderful and amazing things you all have done to make science affordable, efficient, and above all fun. Hope for more products to be coming to the market soon and helping the science and the scientific community."

"The buffer set is very convenient to use and can make plenty of mix&go competent cells and they are also easy and fast to use, greatly shorten my experiment time."

"I found the product super ease to use to obtain competent bacterial stocks. Applicable of more than just E. coli bacteria. It speed up our SOP for molecular cloning, bacterial genome editing and protein production."

"These work very reliably and are super fast and easy to use for students."

"For the price, the Mix and Go! E. coli Transformation Kit is a great tool in advanced biochemistry courses when teaching cloning. Ease of use is equally great!"

"The Mix&Go Transformation kit is so easy to use. The buffer set comes with 2x dilution buffer and one each of wash buffer and competent buffer. You just Mix & Go! We are generally able to reach an efficiency of 10^8 which is reproducible between batches. The cells are very high quality and very effective and we are able to transform nearly any type of plasmid we work with. The kit is a great value for the cost."

"I have used the Mix & Go! E. coli Transformation Kit from Zymo Research for over 6 years now. You can make any E. coli strain you want “heat” shock competent with it. They only take a few hours to prepare and are highly competent and very reproducible. The transformation of plasmids or ligation/InFusion/HiFi/Gibson reactions is very easy and if the antibiotic marker is ampicillin it only takes 5 min (otherwise you will need a regeneration step of 45-60 min)."

"The Mix & Go kit was very simple to use and offered all the materials needed, making it one comprehensive box to use for several reactions. If I could change anything about it, I would rather it be sold in a bulk size, so less single use material is used over the course of ~1000 reactions."

"We regularly use the product for the preparation of competent E.coli. The procedure simple; wash the log-phase E.coli culture with the wash buffer in the kit and then re-suspend it in the competent buffer – that's it! You don't need worry about a complex buffer, multiple washing, and a unreproducible titer!"