![Immunohistochemistry-Paraffin: HSP90 alpha [ac Lys294] Antibody [NBP1-77944] - This antibody was used at 20ug/mL in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/57ac85aaa2584c676de908e1792b7a6b7501915f-400x318.jpg?w=3840&q=75&fit=clip&auto=format)
HSP90 alpha [ac Lys294] Antibody
- Manufacturer
- Novus Biologicals, LLC
- Catalog Number
- NBP1-77944
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Applications
- ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry (Paraffin-Embedded Sections), Western Blotting
![Western Blot: Iigp1 interferon inducible GTPase 1 Antibody [NBP2-03970] - analysis of Iigp1 using Iigp1 polyclonal antibody. Mouse NIH 3T3 lysate in the absence (A) and presence (B) of immunizing peptide probed with Iigp1 antibody at 4 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/3132c3d8b6b6413c767066720b3910844a2d7b5d-211x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Jagged 2 Antibody [NBP2-27420] - Analysis of JAG2 in mouse thymus lysate using 1) prebleed at 1:5000 dilution and 2) this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/60dc12b3a53173d6e0963f9dc5dd6d091edf673f-282x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Lyl1 Antibody [NBP2-27188] - analysis of Lyl1. Lanes 1 and 2 show human HEPG2 cell lysate probed with pre-immune sera (1:5000) and Lyl polyclonal antibody (4 ug/ml), respectively, and lane 3 shows mouse NIH 3T3 cell lysate probed at 4 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4896b9a3afa5678d58b1d11c65d4f63781e61059-340x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: MAGED2 Antibody [NBP2-24694] - Analysis of MAGE-D1/2 in A375 cell lysate using NBP2-24694 at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and Picotect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/108214bd2791197e0b1c0b296732dda665e95de1-226x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: MSX1 Antibody [NBP2-24715] - Analysis of HOX7 expression in formalin-fixed, paraffin embedded normal mouse liver tissue. A: Staining with rabbit IgG (isotype control) at 2.5 ug/ml. B: Staining with rabbit anti-HOX7 at 2.5 ug/ml. C: Higher magnification of B, showing nuclear expression of HOX7.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/24d006153ce86ab4f1b5f916bcbff10d74b5338c-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: NEDD9/CASL/HEF1 Antibody (2G9) [NB100-1699] - Immunofluorescence microscopy using Monoclonal anti-HEF1 antibody (clone 2G9) shows detection of HEF1 localized at focal adhesion sites. The antibody was used at a 1:500 dilution with a 3-sec exposure time. Personal Communication. Elena Pugacheva, Fox Chase Cancer Center, Philadelphia, PA.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/d7bf26d6a82e2d99ba9e83cea53da20c6f32c92c-397x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: Neurotensin Receptor 2 Antibody [NB100-56472] - Analysis of human brain, cerebellum using this antibody at 10 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/9a72faa388af5ee4ffd5d22044c52c3bf54a15ab-400x317.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: NFKBIL1 Antibody [NBP1-70344] - analysis of NFKBIL1. Lanes 1 and 2 show HeLa cell lysate probed with pre-immune sera (1:5000) and NFKBIL1 polyclonal antibody (3 ug/ml), respectively.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/5ac87fe13817c2ddd8f96ae472202e08f12495d3-344x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: NKIRAS1 Antibody (184C278) [NB100-56555] - Analysis of NKIRAS1 using this antibody. Human 293 cell lysate probed with kB-Ras1 antibody at 0.5 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ba1f5d7d2ca05ed4e9c841318bafbd080cbca099-204x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Ornithine Decarboxylase Antibody (SPM565) [NBP2-32888] - Analysis using the Azide and BSA Free version of NBP2-32888. Detection of ODC1 in human placental lysate](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/85ca5fd47d28d1eee44e9ce8d46f4d654cb0a90b-153x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Paxillin [p Tyr118] Antibody [NBP2-24459] - Analysis of phospho Paxillin (Y118) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 118 and 2) an unphosphorylated tyrosine at position 118, using paxillin phospho antibody at 0.025 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/2a9364e453a765b2dd0b42b71c4278b053358f64-380x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: PGLYRP2/PGRP-L Antibody [NBP2-49690] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-PGRP-L at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/1f615616a8cb4badd31f7c660fd2be8653bc3cf7-400x301.jpg?w=3840&q=75&fit=clip&auto=format)
![ELISA: Phosphothreonine Antibody (18F6) [NB600-878] - was tested against BSA conjugates of phosphothreonine (grey squares), phosphotyrosine (black triangles) and phosphoserine (open circles). Each well was coated with 0.1 ug of conjugate. The starting dilution of antibody was 1/1000 and each point on the X axis represents a 2-fold dilution. For detection, an HRP-conjugated Goat anti-Mouse IgG was used as secondary antibody, with a TMB substrate. Absorbance is representred on the Y axis.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/725737c84943867b3d259fc84f965717f7251723-400x263.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Porimin Antibody [NB100-56371] - Western blot analysis of Porimin using NB100-56371 at 2 ug/ml dilution against 20 ug/lane of HL60 cell lysate.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/17fed29fd9ac7f6f88e8b2f102450aa761074833-152x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry/Immunofluorescence: ROBLD3 Antibody [NBP1-71687] - ROBLD3 Antibody was tested at 1:100 in NIH/3T3 cells with FITC (green). Nuclei (Blue) were counterstained with DAPI (blue).](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4a5078738afe6e8507fca91cd7d4a8b1b7f44367-400x294.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: SF3B4 Antibody (3A1) [NBP1-92692] - Western blot of HeLa cell crude extract stained with NBP1-92692. The SF3B4 (3A1) antibody runs with an apparent SDS-PAGE molecular weight 49kDa.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/4ff7756e466e26db0f007da2a654891c8254d86f-159x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunocytochemistry: SUB1 Antibody [NBP1-82454] - Staining of human cell line U-2 OS shows positivity in nucleus & nucleoli.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/b98fc39e6e40fb462585dc0907b9eff964b2cab1-400x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: TAK1 Antibody [NB100-56363] - Western blot analysis of TAK1 in cell lysates from NIH 3T3 cells using NB100-56363 at 1 ug/ml dilution.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/ef391ae4e3c072d1ae2664ba02cdd520e5be6f98-171x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Immunohistochemistry-Paraffin: VANGL2 Antibody [NBP2-24704] - analysis of Van Gogh like-2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/cae2921ed443d390e327200cc55167eb5eb97cad-286x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: VGLL2 Antibody [NBP2-24649] - analysis of VGLL2/VITO1 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/bc70673e2b9952777da1e8a65452c9e7b4deb672-384x400.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: Glycine Receptor Alpha 1 Antibody [NB300-113] - Rat spinal cord showing specific immunolabeling of the ~48k a1- and a2-subunits of the glycine receptor. The labeling was absent from a Rat hippocampal (hipp) lysate as the glycine receptor is not expressed in the hippocampus.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/64bc2219f3777e41d1c91be68744ec31857f507d-400x275.jpg?w=3840&q=75&fit=clip&auto=format)
![Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Tyr1472 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.](https://cdn.sanity.io/images/f5b6mtfn/antibodies-prod/3d2161e97a7aa4e22b34463dbdc7b20a83dfa1d3-400x331.jpg?w=3840&q=75&fit=clip&auto=format)