SPEAR UltraDetect™ NF-L Biomarker Assay Kit
The SPEAR UltraDetect™ NF-L Biomarker Assay Kit lets you quantify neurofilament light with ultra-high precision, low sample volume, and a simple, wash-free workflow. Requires SPEAR UltraDetect™ Reaction Kit. For research use only.
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A universal biomarker for neuronal injury across neurological disorders
Neurofilament Light Chain (NF-L) is a blood-based biomarker serving as a general indicator of axonal damage and neuronal injury across various neurological conditions, including Alzheimer’s disease (AD).
NF-L strongly correlates with neurodegenerative processes and amyloid pathology in AD, as measured by positron emission tomography (PET) and cerebrospinal fluid (CSF) analysis. It also acts as a broad marker of neuronal injury in conditions such as stroke, traumatic brain injury (TBI), and multiple sclerosis (MS).
The Spear UltraDetect NF-L demonstrates Unparalleled Sensitivity and Precision, measuring all healthy samples above fLLOQ with extremely low intra-plate and inter-plate CVs. Researchers leveraging NF-L desire sensitivity and precision – critical immunoassay performance metrics that enable detection of small changes in multi-year longitudinal studies while Clinical trial researcher desire the same performance benefits for applications in enrichment and pharmacodynamic studies.
Confirming this performance with clinical samples – including healthy controls and disease patient samples, the SPEAR UltraDetect NF-L assay quantified 100% of patient samples was able to achieve a mean CV% of 3.2%.
SPEAR UltraDetect Neurofilament light exceptional precision with F.A.S.T. automated workflow
This application note focuses on SPEAR technology and its enhanced protein detection sensitivity and specificity compared to conventional immunoassays for detecting Neurofilament light in sub-microliter samples of serum or plasma. Integrated with the Formulatrix F.A.S.T. system, the wash-free assay workflow is further streamlined, reducing hands-on time to under 20 minutes and achieving remarkable precision (mean CV of 3.1%).
SPEAR UltraDetect brochure
SPEAR UltraDetect™ is an ultra-sensitive, homogeneous immunoassay platform designed for reliable detection of low-abundance protein biomarkers. Powered by SPEAR’s unique two-factor authentication mechanism and standard qPCR readout, UltraDetect assays deliver exceptional sensitivity, specificity, and precision in a fully wash-free workflow using as little as 1 µL of sample. The solution enables robust biomarker quantification for neurological and clinical research applications with simplified workflows and broad instrument compatibility.
Download this brochure to discover the fundamentals of SPEAR Technology, key features of SPEAR UltraDetect Assays, and a comprehensive summary of the complete SPEAR Solution.
Ultra-sensitive immunoassay tech advances Alzheimer’s blood-based biomarker detection
SPEAR overcomes immunoassay complexity and sensitivity limitations in Alzheimer’s and neurodegenerative biomarker research with a homogeneous, wash-free format
