SURFE²R N1 by Nanion Technologies GmbH

The SURFE²R N1 works with reusable sensor chips on which the membranes containing the protein of interest are adsorbed. In contrast to conventional patch-clamp electrophysiology the proteins of electrogenic transporters and pumps have low turnover rates compared to ion channels. The SURFE²R N1 technology compensates for that with a large sensor size which allows for the measurement of up to 10⁹  transporters at the same time to yield the best signal to noise ratio.  

Any kind of membrane preparation containing the protein of interest can be used for measurements, e.g. membrane vesicles after cell disruption or proteoliposomes after reconstitution of purified proteins.

The SURFE²R N1 allows for determination of parameters such as EC₅₀ or IC₅₀. Since the time resolution of solution exchange is state-of-the-art, not only slow transport can be measured, but also fast binding reactions can be assayed and rate constants can be determined.  

Features:  

• More than 100 targets validated, 100 peer reviewed papers using SSM-based electrophysiology
• Analysis of transporters, pumps and ligand gated channels
• Measure even electroneutral exchangers and sugar binding
• Use purified membranes and vesicles from cells or proteoliposomes
• Label-free electrical measurements
• Requires only 0.1 – 1 µg protein per sensor. This is sufficient for up to 100 experiments.
• Real-time data with high time resolution, not single point read-out
• High signal amplification compared to patch-clamp
• Fast binding kinetics can be resolved
• Fully-automated recordings with 52 different test solutions possible
• 150 data points per day