Raptor Polar X LC Columns

This comprehensive guide from Restek provides everything analytical labs need for accurate, efficient, and compliant PFAS testing. Covering certified reference materials (CRMs), LC and GC columns, sample preparation tools, and air/gas sampling equipment, it’s a one-stop resource for labs working with water, food, environmental, and industrial samples. Featuring PFAS 3 and PFAS 4 mixes, method details, and product specifications, this catalog supports both routine and advanced PFAS workflows.

Due to their ubiquitous occurrence in aquatic environments, measuring ultrashort-chain per- and polyfluoroalkyl substances (PFAS) in various source waters to monitor their presence and the potential for human exposure has become very important. However, with carbon chain lengths of less than C4, these small, highly polar compounds are difficult to analyze using standard PFAS tests that are based on reversed-phased liquid chromatography (RPLC). Restek Corporation presents an accurate, reliable analytical LC-MS/MS method for PFAS in water that was developed to specifically quantify C1 to C4 PFAS in both potable and non-potable sources. A direct injection workflow was implemented to simplify the testing process and to avoid potential contamination originating from poor sample preparation procedures.

In this poster, Restek describes a simple workflow that was developed and implemented for the accurate quantification of a large panel of 45 amino acids in human plasma. A fast chromatographic analysis (13 minutes) was achieved using a unique hybrid HILIC/ion exchange column for high-throughput amino acids profiling in plasma.

In this application note, Restek demonstrates a fast and simple LC-MS/MS method that was established for comprehensive PFAS analysis that includes C2, C3, C4, C6, C8, and alternative PFAS. Even longer-chain PFAS are possible, too. This method can be applied to both potable and non-potable water analysis and provides convenient setup and high throughput for labs interested in adding ultrashort-chain compounds to an existing PFAS assay.

In this application note, Restek describes how its Raptor Polar X stationary phase is specifically designed to selectively retain polar analytes through a balance of two retention mechanisms. This unique hybrid phase is ideal for analyzing a wide variety of polar compounds, especially when coupled with mass spectrometry.

In this application note, Restek details a method for glyphosate in water in which an anion-exchange mechanism in the column provides enough retention under the initial mobile phase conditions to allow large volumes (500 µL) of bottled water to be injected, which is a very simple way to improve low-level sensitivity.

To ensure that nutritional requirements are met, reliable analytical methods are needed for the accurate determination of free amino acids in infant formulas.

In the approach shown here, underivatized amino acids can be measured directly—following a single-step sample preparation procedure—using a Raptor Polar X column paired with an MS/MS detector. Download the full application note below to learn more.

In this application note, Shimadzu demonstrates how the LCMS-9030 promises excellent mass accuracy for N-linked glycans on several proteins and explains how Restek's Raptor Polar X, an innovative hybrid ion exchange/HILIC column, is an ideal column for glycans.

In this video, Restek describes how to properly condition your LC column. Clean, stable baselines are critical to good liquid chromatography, and properly conditioning your LC column is a helpful way to ensure a good baseline. You’ll need to flush out the storage solvent and equilibrate the column to your solvent mixture, and you may need to use an intermediate solvent to ensure complete mixing and flushing.

In this video, Restek discusses LC column cleaning and regeneration. Watch to learn the steps you can take to keep your column clean and help restore some performance to columns and extend their lifetime.

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