Assessing single membrane-impermeable dyes for nuclei assessment derived from kidney FFPE tissue using the LUNA-FX7™ automated cell counter

This study evaluates membrane-impermeable fluorescent dyes, including PI, NucSpot 470, and YOYO-1, to determine their effectiveness in staining nuclei isolated from kidney FFPE samples using the LUNA-FX7™ automated cell counter from Logos Biosystems. By optimizing dye selection and staining protocols, the study aims to improve the reliability of nuclei quality assessments in FFPE tissues, ensuring accurate results and minimizing artifacts caused by debris